HTS Implementation
Southern Research Institute's High Throughput Screening Center is designed for rapid, target-specific analysis using robotics to evaluate large compound libraries. The staff is experienced in moving bench top assays to the robotics platform and has run many target and cell-based screens. Our core assay system is equipped with various readers that enable us to develop assays based on your choice of endpoint detection methods. One of our unique capabilities is our ability to run HTS screens with BSL-2 or BSL-3 pathogens. Our current micro-plate capabilities include 96-, 384-, and 1536-well formats.
Our recent HTS assays include:
- Inhibitors of the enzyme pantothenate synthetase, an important bacterial virulence factor, assayed spectrophotometrically.
- Viral inhibition assessment through Cytopathic effect (CPE) assays using ATP as a marker for cell viability (SARS-CoV and Influenza) or through specific molecular targets/characteristics (HIV) driving reporter genes.
- Inducers of the excitatory amino acid transporter-2 (EAAT-2) using a cell based luciferase reporter.
- Growth inhibition of cells transformed with the DNA binding transcription factor GKLF gene using the tetrazolium compound MTS.
Southern Research has a multi-million dollar investment in its HTS center. The space and equipment are described in the HTS Equipment page.
