E. Lucile White
Ms. Lucile White is an expert in the development of methods for the purification, characterization, and assay of enzymes. She has over 30 years of experience working with enzymes representative of all six main enzyme classes and is familiar with most methods used in following enzyme reactions - spectrophotometric, fluorescence, continuous titration with pH-stat, and radioactive tracers.
During this time she has worked with the five isozymes of lactate dehydrogenase, 3-phosphoglycerate kinase, human chorionic gonadotropin (HCG) and its two subunits, tissue plasminogen activator (TPA), urokinase, plasmin, viral reverse transcriptases, HIV-1 RNase H, DNA polymerase α, β, γ, and δ, S-adenosylhomocysteine hydrolase, adenosylsuccinate synthetase, adenosylsuccinate lyase, tyrosinase, phospholipase A2, elastase, collagenase, nucleotidases, ornithine decarboxylase, S-adenosylmethionine decarboxylase, aromatase, glucose-6-phosphate dehydrogenase, hypoxanthine-guanine phosphoribosyltransferase, protein kinase C, luciferase, and receptor tyrosine kinases (erbB-2 and epidermal growth factor receptor).
In addition, she has published on the purification of synthetic peptides by means of counter-current chromatography and the analysis of fluorescence spectra of luteinizing hormone releasing hormone (LHRH). Her work at Southern Research has been primarily aimed at testing the effects of drugs on target enzymes, including contract and grant work. In 2004, Ms. White assumed responsibility for managing the High-Throughput Screening Center at Southern Research where she oversees the screening of large compound libraries against enzyme, protein, and cell-based assays. The HTS Center works on both federally funded grants and contracts as well as with commercial clients.
